RT-PCR Analysis of Caucasian and Mugla Honey Bees by SNP Markers of Chalkbrood Disease
نویسندگان
چکیده
Two different honey bee subspecies’ genotypes obtained from Ordu, Apiculture Research Institute were analyzed by SNP markers using Real-Time PCR-HRM. Genomic DNA samples analysed with 10 primers those used for identification of chalkbrood disease resistance genes and two genom sequencing. Result analyses, four (AMB-00858574, AMB-01151447, AMB00631190, AMB-00686140) amplified in Caucasian six AMB-00612262, AMB-00631190, AMB-00674355, Mugla bee. Four amplicons are similar this subspecies. electrophoresis five (AMB00858574, form a band bee, seven AMB00612262, AMB-00902548, As result study, Muğla honeybee evaluability as key subspecies was emerged the capacity association single nucleotide polymorphisms to important country Apis mellifera RT-PCR HRM analysis.
منابع مشابه
Chalkbrood disease in honey bees.
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ژورنال
عنوان ژورنال: Uluslararas? Tar?m ve Yaban Hayat? Bilimleri Dergisi
سال: 2023
ISSN: ['2149-8245']
DOI: https://doi.org/10.24180/ijaws.1185587